MCADD+Materials+and+Methods

=Websites/Applications used:=

[|National Center for Biotechnology Information (NCBI)]
 * Accessed between September and October 2017
 * Searched for Human Medium-chain acyl-CoA Dehydrogenase and Human ACADM gene
 * Acquired both nucleotide and protein sequences along with their accession numbers
 * BLAST engine the human sequences to acquire sequences of other species for both MCAD enzyme and ACADM gene



Molecular Evolutionary Genetics Analysis (MEGA7)
 * Accessed between September and October 2017
 * Performed sequence alignment (ClusterW method) for both nucleotide and protein sequences
 * All positions containing gaps and missing data were eliminated.
 * Constructed phylogeny trees (Maximum likelihood, neighbor-joining, Minimum likelihood)

MEGA 7 Parameters for cluster and phylogeny bootstrap value

[|ExPASy Bioinformatics Resource Portal]
 * Accessed October and November 2017
 * Resource portal for bioinformatic related tools:
 * Searched for protein structure analysis tools
 * Protein Model Portal was referred as a tool

[|Protein Model Portal]
 * Accessed October and November 2017
 * Structure analysis of MCAD was searched for
 * Structure analysis MCAD
 * //Homo Sapiens// wild-type MCAD
 * //Homo Sapiens// MCAD K304E Mutant (most prevalent)
 * //Mus Musculus// MCAD

[|Protein Data Bank Portal]
 * Accessed November 2017
 * Structure analysis of MCAD K304E mutant

STRING Database
 * Accessed November 2017
 * Database of known and predicted protein-protein interactions.
 * Interactions include direct (physical) and indirect (functional) associations; they stem from computational prediction, from knowledge transfer between organism, and from interactions aggregated from other (primary) databases


 * Search //Homo sapiens & Mus musculus// ACADM



Sequences obtained for ACADM gene and MCAD enzyme:

= = =**Directory:**= MCADD homepage @Primary Research Results Discussion References