CFTRResearch

Bibliography (APA) Lee, Tim W. et al. (2005, April 1). Novel Molecular Approaches to Cystic Fibrosis. //Biochem Journal//, Volume 387. Pages 1-15. Retrieved October 30, 2012, .

Palca, Joe. (1994, June 1). The Promise of a Cure. //Discover Magazine, June 1994//. Retrieved October 29 2012, .

Published June 1, 2011. What Are the Signs and Symptoms of Cystic Fibrosis? //Nation Heart Lung and Blood Institute.// Retrieved November 10, 2012. From <@http://www.nhlbi.nih.gov/health/health-topics/topics/cf/signs.html>.

Rowe, Steven M (2011, August 1). A Breath Of Fresh Air. //Scientific American//, Volume305, Issue 2. Retrieved November 2, 2012, 

Rowe SM, Miller S, Sorscher EJ (May 2005). [|"Cystic fibrosis"]. //N. Engl. J. Med.// **352** (19): 1992–2001.

http://www.hopkinscf.org/main/whatiscf/science_cftrmut.html

CFTR classes picture - http://carolguze.com/text/442-11-clinical_genetics.shtml

Bioinformatics Tools **BLASTp** --- Basic Local Alignment Search Tool (protein) was used to find the similarities and differences among different species using amino acid sequences.
 * ClustalW Alignment** - ClustalW is a general purpose multiple sequence alignment program for DNA or proteins. This is a process available through MEGA 5 software.

**Geneious**--- This software (freely available) was used to obtain protein sequences of the CFTR gene found in many species. Geneious uses all available data from NCBI, searching through, literally, billions of sequences to obtain results. The sequence for humans was found and compared to the other 9 species sharing similar sequences.

**MEGA 5** --- This is a tool to conduct sequence alignment to help create phylogenetic trees. Sequences were imported from Geneious for analysis in MEGA using a ClustalW alignment to find similar and dissimilar characters.
 * OMIM** --- This is a database of human genes and genetic disorders (omim.org).

To investigate if the CFTR gene is conserved in other species, a program called Geneious was used to locate the human version of the gene. Geneious uses all available data from NCBI, searching through, literally, billions of sequences to obtain results. After locating the necessary files, I export the sequences into MEGA to align the sequences. The alignment of sequences is done through ClustalW process to find similar and dissimilar characters. Geneious uses all available data from NCBI, searching through, literally, billions of sequences to obtain results.


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