Pancreatic+Cancer+Methods

METHODS The focus of the research was on the Slit-Robo genes. The signaling protein sequence were found in research databases. The sequences were then put through BLAST to gather other forms found within humans and other species. BLAST is a tool that can use protein or cellular sequences and find similar sequences based on their ability to align together. Slit-Robo signaling sequences were gathered among various species. Proteins that all have to do with the GTP-ase activating and interaction component of the Slit-Robo signaling pathway. The proteins were then aligned by Clustal W in the tool, MEGA. MEGA is a tool that is able to take sequences and put them into visual alignments. It also enables phylogenic trees to be made with the sequences which show the level of heterogeneity between sequences. There are various forms of phylogenic trees that can be constructed. Specific parameters can be added to bring a greater degree of specificity to sequences being analyzed. In this project the--- tree was used using the Bootstrap method as an added parameter.  KEGG pathway- will assist in research of the effects felt in the pathway of the mutated genes, Human Genome Browser- will assist in researching the genome for pancreatic cancer

Pancreatic Cancer Data Pancreatic Cancer Background Pancreatic Cancer Bibliography