HGPS+Methods

Methods

BLAST is a comprehensive bioinformatics tool which is available to the public and enables the comparison of a variety (nucleotide, protein, amino acid, etc.) of sequences or genes. Anyone from a college student to a genomic research team can find this tool useful. I used BLAST to both identify the sequenced LMNA gene and Lamin A protein among different species and compare their similarities.
 * 1.) BLAST (Basic Local Alignment Search Tool) **

OMIM is a complete resource for all inquiries regarding diseases that have a known genetic component. I used OMIM to research the location of my gene of interest, to view a quick snapshot of all the work previously done on the Lamin A gene and on HGPS.
 * 2.) OMIM (Online Mendelian Inheritance in Man) **

MEGA is a great tool to use for a visual rendering of comparative sequence analysis. MEGA allows users to see how genes have evolved over time within a variety of species. I used MEGA to compare my gene of interest between many species. I was able to see which species were most closely and most distantly related in terms of my specific research focus. I used MEGA to create phylogenetic trees.
 * 3.) MEGA (Molecular Evolutionary Genetics Analysis) **

PDB is a database of proteins and nucleic acids that can be seen in their 3D structure. It allows users to visualize proteins from multiple perspectives.
 * 4.)PDB (Protein Data Bank) **

Cn3D is another visual tool used to see proteins and nucleic acids in 3D, it is vastly more interactive and customizable than PDB. Using the PDB id for my gene, I used Cn3D to pinpoint the C-terminus of my protein (where the mutation for HGPS exits).
 * 5.)Cn3D **

STRING is a public database where protein-proteins interactions can be searched and analyzed.
 * 6.) STRING **

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