Ebola+Virus+Methods

Twenty three genomes were aligned for analysis. There were six Reston Virus genomes and seventeen Ebola genomes, two of which were confirmed infected-animal //Zaire ebolavirus//. From the fifteen genomes, twelve were from the population of interest in the Nigerian outbreak, two were from the Liberia sub-lineage that introduced the virus to Nigeria and the final genome was from the earliest case with genomic data in the entire West African outbreak. Accession numbers and description of each genome is organized in Figure 1.  Figure 1. ACCESSSION NUMBERS FOR GENOMES OF INTEREST AND CORRESPONDING PROTEIN SEQUENCES

Viral genomes were obtained from the GenBank and Virus Pathogen Resource database. Sequences were downloaded into a FASTA file and configured to a Mega7 workbench. The entire viral genome for each case was aligned in silico using the ClusterW method in Mega7. The parameters of the alignment are shown in Figure 2.



After aligning the complete genomes, phylogenetic tree were generated using three methods, Maximum Likelihood, Neighbor Joining and Minimum Evolution. All trees were completed with Boot Strap Analysis in the parameters. The complete parameters for the phylogenetic trees are outlined in Figure 3.



There were five proteins of interests: VP24, VP30, VP40, NP and GP. The protein sequences were obtained from the GenBank with the referenced and corresponding genome. The protein sequences were aligned by protein type using the same parameters as the genome alignment and are described in Figure 4.



Phylogenetic trees were generated for each protein sequence using the same parameters as the genome sequence and are outlined in Figure 5.

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Introduction Primary Research Data and Results Discussion References