Phylogenetic+Tree+HGPS

Phylogenetic Tree

To further investigate the //LMNA// gene mutation a phylogenetic tree was generated using the database MEGA 5. From this, evolutionary relationships between species, their common ancestry and how their genes are conserved could be analyzed. To do this, a BLASTp for a specific protein sequence (//Homo sapien//- Full Prelamin-A precursor) was performed in the program Geneious. Once a batch export transferred the sequences to MEGA it was aligned by clustal W.



From this alignment a Maximum Likelihood phylogenetic tree was constructed using a bootstrap analysis. For a tree to be reliable the bootstrap values must be at least 70. Analyzing the //LMNA// gene mutation in relation to other species such as //Pan troglodytes// (chimpanzee), //Nomascus leucogenys// (northern white-cheeked gibbon), //Rattus norvegicus// (norwegian rat) it was observed that the outgroup " //Homo sapien// full prelamin A precursor" was closely related to all the ingroups except //Canis lupus familiarius// (dog) and //Equus caballus// (horse).



Determining common ancestry from a phylogenetic tree and knowing evolutionary relationships aids in choosing a model organism. This model organism is used to study, for example, a gene of interest in a controlled setting where it can be manipulated to understand the disease, disorder, mutation further.

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