A1AT+Materials+and+Methods

=Materials & Methods= Disease: Alpha-1 Antitrypsin Disorder Gene: SERPIN1A Protein: A1AT

=Bioinformatic Tools Used=
 * 1) **NCBI **: Website used to determine disease gene and protein sequence identification.
 * 2) **BLAST **: This tool was used to compare the human nucleotide and amino acid sequence to that of other animal species (95% overlap or better required).
 * 3) **MEGA **: This tool was used to align the nucleotide and amino acid sequences of the species selected from Blast. In addition, phylogenic trees were produced and likeness relationships were identified.


 * Genes and Proteins **
 * **Nucleotide** |||||| **Protein** ||
 * ** Scientific Name ** || ** NCBI # ** || ** Common Name ** || ** Scientific Name ** || ** NCBI # ** || ** Common Name ** ||
 * Homo Sapiens || K01396.1 || Human || Homo Sapiens || P01009.3 || Human ||
 * Gorilla gorilla gorilla || XM_019009747.1 || Gorilla || Gorilla gorilla gorilla || XP_004055679.1 || Gorilla ||
 * Pan tronglodytes || XM_009428362.2 || Common Chimpanzee || Pan tronglodytes || XP_003314546.1 || Common Chimpanzee ||
 * Pan paniscus || XM_008958252.1 || Pygmy Chimpanzee || Pan paniscus || XP_003832864.1 || Pygmy Chimpanzee ||
 * Pongo abelii || XM_009249430.1 || Sumatran Orangutan || Pongo abelii || XP_009247699.1 || Sumatran Orangutan ||
 * Nomascus leucogenys || XM_012498081.1 || Northern White-Cheeked Gibbon || Nomascus leucogenys || XP_003260972.1 || Northern White-Cheeked Gibbon ||
 * Papio anubis || XM_009212185.3 || Olive Baboon || Cercocebus atys || XP_011939206.1 || Sooty Mangabey (Old World Monkey) ||
 * Chlorocebus Sabaeus || XM_007987700.1 || Green Monkey || Chlorocebus Sabaeus || XP_007985889.1 || Green Monkey ||
 * Macaca mulatta || XM_015144331.1 || Rhesus Macaque || Macaca nemestrina || XP_011715420.1 || Pigtail Macaque ||
 * Canis lupus || AB287507.1 || Gray Wolf || Microcebus murinus || XP_012594085.1 || Gray Mouse Lemur ||

= BLAST Parameters = Default parameters were used for BLAST.

[[image:MEGA Nucleotide Parameters.PNG width="529" height="551"]]

 * Mega Maximum Likelihood Protein Tree Parameters **

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