Neurofibromatosis+Type+1+Methods

Methods:

 Bioinformatics Tools used.

 BLASTp- utilized to determine if the gene of interest is present in any other species and if it maintains the same functions. Used it to find the similarities and differences among different species using the amino acid sequence.

 MEGA- utilized this tool to conduct sequence alignment in order to create phylogenetic trees to better visualize the relationships between sequences that were obtained from BLASTp searches for the protein of interest. Bootstrap values of

 HAPMAP- utilized this tool to determine the prevalence of the disease and the gene across the globe.

 KEGG- utilized this tool to understand the molecular pathway and biological processes associated with the gene of interest.

Sequence Analysis obtained from BLASTp and Aligned using MEGA. 

 Sequences above:
 * Number || Organism ||
 * 1 || Human ||
 * 2 || Human isotope ||
 * 3 || <span style="font-family: Georgia,serif; font-size: 110%;">Crab Eating Macaque ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">4 || <span style="font-family: Georgia,serif; font-size: 110%;">Partial sequence of Crab Eating Macaque ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">5 || <span style="font-family: Georgia,serif; font-size: 110%;">Rhesus Macaque ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">6 || <span style="font-family: Georgia,serif; font-size: 110%;">Isotope of Human ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">7 || <span style="font-family: Georgia,serif; font-size: 110%;">Isotope of Human ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">8 || <span style="font-family: Georgia,serif; font-size: 110%;">Black Flying Fox ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">9 || <span style="font-family: Georgia,serif; font-size: 110%;">House Mouse ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">10 || <span style="font-family: Georgia,serif; font-size: 110%;">Brown Rat ||
 * <span style="font-family: Georgia,serif; font-size: 110%;">11 || <span style="font-family: Georgia,serif; font-size: 110%;">Isotope of House Mouse ||

<span style="font-family: Georgia,serif; font-size: 110%;">Phylogenetic Tree established using MEGA. UPMGA Tree



<span style="font-family: Georgia,serif; font-size: 110%;">Utilized this tree instead of others produced because it had the most boot strap values above 60 which indicate a high degree of similarity. From the figure shown it is easy to visualize the complexity of the genotypes among species as well as individual human beings. The value of 59 between the Human isotope ( X3) and the MRCA of the other human isotopes and the Crab Eating Macaque is a clear indication of the variability of this disease.