CAD+-+Methods

A literature search was performed and primary research articles were identified. Secondary sources and meta-analyses were also reviewed for context. The CDKN2A gene was identified as a gene of interest and research on it using many genomics tools was performed. The gene sequence was found using NCBI Gene. The sequence was then run through NCBI BLASTn to identify similarity to other sequences across species. Many species were identified as having the same gene as is seen in humans. Species were chosen and those sequences were isolated. They were then aligned using MEGA 7.0. The alignments were then used to create phylogenetic trees to examine the evolutionary relationships between each species. The gene sequence was also used to explore protein structure and protein interactions related to CDKN2A. STRING provided relevant protein interactions. NCBI Structure provided the structure of a primary protein that CDKN2A interacts with.







__All tools used:__ NCBI Gene Search Term: CDKN2A https://www.ncbi.nlm.nih.gov/gene

NCBI BLASTn Search Term: CDKN2A Sequence https://blast.ncbi.nlm.nih.gov/Blast.cgi

MEGA 7.0 Used to align sequences of CDKN2A nucleotides and predicted proteins Used to create phylogenetic trees for nucleotide and protein http://www.megasoftware.net/

STRING Used to visualize protein and gene interactions relating to CDKN2A Search Term: CDKN2A http://string-db.org/

NCBI Structure Used to develop a structural model of a common protein coded for by CDKN2A Search Term: CDKN2A https://www.ncbi.nlm.nih.gov/Structure/

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Introduction Primary Research Data and Results Discussion References