A1AT+Results

Mega Alignments Nucleotide Alignment

Protein Alignment

Mega Phylogenic Trees Nucleotide Maximum Parsimony Tree The nucleotide maximum parsimony tree demonstrates a clear separation in the center making three (3) distinct groupings. Chlorocebus sabaeus is a distinct out-group, and its branch has a zero value for phylogenic linkage to the rest of the species. Papio anubis and macaca mulatta are 90% similar to each other, but they show a clear distinction from the chlorocebus sabaeus, and from the other seven (7) species. The last seven (7) are evidence of the most phylogenic similarity. The pan tronglodytes and pan paniscus are strongly related with 80% similarity to each other. These species connect to the human alpha-1 antitrypsin nucleotide, and then the gorilla gorilla gorilla, with values of 94% and 97%, respectively. The pongo abelii and nomascus leucogeny are the next branches of the tree; however, with values of 41% and 53%, respectively, the evidence for similarity between these two (2) species the previous four (4) is weak. Finally, the branch for the canis lupus, connected with the six (6) species above, shows the strongest phylogenic comparison, with a value of 100%.



Protein Maximum Likelihood Tree The protein maximum likelihood tree demonstrates a clear separation in the center making three (3) distinct groupings. Microcebus murinus is a distinct out group and its branch has a zero value for phylogenic linkage to the rest of the species. Cercocebus atys and macaca nemsestrina show 80% similarity and connect to chlorocebus sabaeus with a 94% similarity; however, this grouping is clearly separate from the microcebus murinus, and from the group of six (6) species). The pan tronglodytes and pan paniscus are strongly related with 82% similarity to each other. These species do not show great similarity to the human alpha-1 antitrypsin protein, but then have high phylogenic similarity to gorilla gorilla gorilla, with values of 62% and 99%, respectively. The pongo abelii and nomascus leucogeny are the next branches of the tree, with values of 76% and 89%, respectively.

3D Structure Images

The 3D images rendered by UniProt and Biozentrum Swiss Model are similar, yet quite different. Both show distinct areas of alpha helices and beta sheets; however, the UniProt model is quite basic in comparison to the Swiss model. The UniProt shows a single alpha helix on the left side (green), and only a handful of beta sheets (multiple colors with arrows). The Swiss model has far greater detail with multiple alpha helices intertwining with beta sheets, creating a much more interlocking and complicated looking structure than the UniProt model.

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